Network Pharmacology Based and Molecular Docking Prediction of the Active Ingredients and Mechanism of Ziziphi spinosae semen-Schisandrae chinensis fructus for Application in Insomnia Treatment

To investigate the molecular mechanism of Ziziphi spinosae semen and Schisandrae chinensis fructus in treatment insomnia is objective study. First, compounds corresponding target genes were obtained by traditional Chinese medicine systems pharmacology database analysis platform associated with GeneCards database. The results imported into Cytoscape software to construct a network (Herbal-active compound- Target-disease related network). Then, gene ontology Kyoto encyclopedia genomes enrichment analyses performed for common disease (Target-disease genes). Finally, key identified docking was verified Autodock software. A total 21 components 43 screened 41 target-disease identified. 191 functional terms 26 pathways show that neuroactive ligand-receptor interactions including cholinergic, norepinephrine dopaminergic signals play an important role insomnia. Solute carrier family 6 member 3, cholinergic receptor nicotinic alpha 7 subunit, nuclear subfamily 3 group C 1, 2 estrogen muscarinic acetylcholinesterase progesterone as genes, their showed all could stably bind active pockets. Through these techniques, multi-component synergistic can be elucidated.